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KMID : 0364820140500020169
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.169 ~ p.172
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The Interaction between HCV-Infected huh7.5 Cells and HCV-Specific T Cells
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Kang Hyo-Jeung
Cho Hyo-Sun
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Abstract
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Recently, Hepatitis C virus (HCV) replication system has been established using human hepatoma cells (huh cell)
and a variety of HCV clones. In this study, we established an infectious HCV replication system using huh7.5 cells
and J6/JFH1 clone (genotype 2a). In addition, we investigated the antigen presentation capability of HCV-infected
huh7.5 cells to HCV-specific T cells. Interestingly, HCV-infected huh7.5 cells were not capable of activating
HCV-specific T cells. However, huh7.5 cells stimulated by exogenous HCV peptide were able to activate
HCV-specific T cells, which was shown to produce TNF-α and IFN-γ. We further examined if HCV infection has an
inhibitory effect on the expression of MHC class I molecule of huh7.5 cells. We found that HCV infection did not
change the expression level of MHC class I molecule on huh7.5 cells.
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KEYWORD
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HCV-specific T cells, hepatoma cell 7.5 (huh7.5), IFN-γ, J6/JFH1 clone, TNF-α
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